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Research Interests
Professor Tegeder's research deals with the molecular identification and functional characterization of transport processes controlling the partitioning of organic molecules (e.g. amino acids or sucrose) in plants. A major focus is to understand the mechanisms regulating organic nitrogen transfer from the location of production in roots or leaves to sink organs such as developing reproductive structures and seeds. The isolation and characterization of transport systems using functional genomic approaches will provide the tools required for biotechnological applications in plants. The ultimate goal is to develop strategies aimed at guaranteeing productivity and improving nutritional quality of important crop plants.
Research areas include: (a) Transporter gene identification using bioinformatics (b) Functional complementation of yeast mutants to isolate novel transporters, and subsequent kinetic analysis using heterologous expression systems (c) Expression analysis using functional genomic and proteomic techniques to understand the positioning in the biochemical networks and mechanisms controlling the distribution of transport systems. (d) Functional characterization of transporters in plants by mutant analysis to evaluate their physiological role and effects of modification
Representative Publications
Foster J., Lee Y.H. and Tegeder M. (2008) Distinct expression of members of the LHT amino acid transporter family in flowers indicates specific roles in plant reproduction. Sex Plant Reprod, published online.
Tan Q., Grennan A., Rentsch D. and Tegeder M. (2008) Isolation and characterization of an amino acid transporter from French bean (Phaseolus vulgaris L.). Plant Sci, 174, 348-356.
Tegeder M., Tan Q., Grennan A. and Patrick J.W. (2007) Amino acid transporter expression and localisation studies in pea (Pisum sativum L.). Functional Plant Biol, 34, 1019-1028.
Rentsch D., Schmidt S. and Tegeder M. (2007). Transporters for uptake and allocation of organic nitrogen compounds in plants. FEBS Letters, 581, 2281-2289.
Lee Y.H., Foster J., Chen J., Voll L.M., Weber A.P.M. and Tegeder M. (2007). AAP1 transports uncharged amino acids into roots of Arabidopsis. Plant J. 50, 305-319.
Pélissier H.C. and Tegeder M. (2007) PvUPS1 plays a role in source-sink transport of allantoin in French bean (Phaseolus vulgaris). Functional Plant Biol, 34, 282-291.
Tegeder M. and Weber A.P.M. (2006) Metabolite transporters in the control of plant primary metabolism. In Control of primary metabolism in plants (Plaxton, W. and McManus, M., eds.), Oxford: Blackwell Publishing. Annu. Plant Rev. 22, 85-120.
Lee Y.-H. and Tegeder M. (2004) Selective expression of a novel high affinity transport system for acidic and neutral amino acids in the tapetum cells of Arabidopsis flowers. Plant J, 40, 60-74.
Pelissier H. C., Frerich A., Desimone M., Schumacher M., and Tegeder M. (2004) PvUPS1, an allantoin transporter in nodulated roots of French bean. Plant Physiol 134, 664-675.
Koch W., Kwart M., Laubner M. , Heineke D., Stransky H., Frommer W.B. and Tegeder M. (2003) Reduced amino acid content in transgenic potato tubers due to antisense inhibition of the leaf H+/amino acid symporter StAAP1. Plant J 33, 211-20.
Lalonde S., Tegeder M., Thorne-Holst M., Frommer W.B. and Patrick J.W. (2003) Phloem loading and unloading of sugars and amino acids. Plant Cell Environ 26, 37-56.
Okumoto S., Schmidt R., Tegeder M., Fischer W.N., Rentsch D, Frommer W.B., Koch W. (2002) High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis. J Biol Chem 277, 45338-46.
Wipf D., Ludewig U., Tegeder M., Rentsch D., Koch W. and Frommer W.B. (2002) Amino acid/neurotransmitter transporters are highly conserved between fungi, plants, and animals. Trends Biochem Sci 27, 139-47.
Wipf D., Benjdia M., Tegeder M. and Frommer W.B. (2002). Characterization of a general amino acid permease from Hebeloma cylindrosporum by functional complementation of a yeast mutant. FEBS Letters 528, 119-124.
Rosche E., Blackmore D., Tegeder M., Richardson T., Schroeder H., Higgins T.J.V., Frommer W.B., Offler C.E. and Patrick J.W. (2002) Seed-specific overexpression of a potato sucrose transporter increases sucrose uptake and growth rates of developing pea cotyledons. Plant J 30, 165-175.
Tegeder M., Offler C.E., Frommer W.B. and Patrick J.W. (2000) Amino acid transporters are localized to transfer cells of developing pea seeds. Plant Physiol 122, 319-326.
Tegeder M., Thomas M., Hetherington L., Wang X.-D., Offler C.E. and Patrick J.W. (2000) Genotypic differences in seed growth rates of Phaseolus vulgaris L. Factors contributing to cotyledon sink activity and sink size. Aust J Plant Physiol 27, 119-29.
Tegeder M., Wang X.-D, Frommer W.B, Offler C.E, and Patrick J.W. (1999). Sucrose transport into developing seeds of Pisum sativum L. Plant J 18, 151-61.
Zenkteler M., Tegeder M., and Schieder O. (1998). Embryological studies of reciprocal crosses between Vicia faba and Vicia narbonensis. Acta Soc Bot Pol 67, 37-43.
Gerdemann-Knoerck M. and Tegeder M. (1997). Compendium of genetically modified plants which are relevant for field releases: Brassicacea, Beta vulgaris, Linum usitatissimum. pp 1-221. Environmental Federal Office, Berlin. Textbook.
Tegeder M., Kohn H., Nibbe M., Schieder O. and Pickardt T. (1996). Plant regeneration from protoplasts of Vicia narbonensis via somatic embryogenesis and shoot organogenesis. Plant Cell Rep 16, 22-25.
Tegeder M., Gebhardt D., Schieder O. and Pickardt T. (1995). Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos. Plant Cell Rep 15, 164-169.
Tegeder M., Schieder O. and Pickardt T. (1995). Breakthrough in the plant regeneration from protoplasts of Vicia faba and V. narbonensis. Current Issue Plant Cell Biol, Kluwer, London, pp 75-80.