Our laboratory is involved in basic and applied genetic research in fishes, especially trout and salmon. We utilize a variety of chromosome set manipulation procedures to trout and salmon to increase our understanding of basic genetics in these fishes and for their genetic improvement. Androgenesis and gynogenesis involve producing offspring with both chromosome sets from one parent and can be useful for producing inbred lines, gene mapping and other studies. Triploids can also be readily produced and are of interest because they are sterile and because triploid interspecific hybrids have been demonstrated frequently to be much more viable than diploid hybrids. Our laboratory is also analyzing DNA polymorphisms in trout and salmon with the goals of developing improved genetic maps, identifying markers for conservation programs and identifying single genes with significant physiological effects.
Evenhuis, J.P., G.D. Wiens, P.Wheeler, T.J. Welch, S.E. LaPatra and G.H. Thorgaard, 2014. Transfer of serum and cells from Yersinia ruckeri vaccinated doubled-haploid Hot Creek rainbow trout into outcross F1 progeny elucidates mechanisms of vaccine-induced protection. Dev. Comp. Immunol. 44: 145-151.
Christensen, K.A., J.P. Brunelli, P.A.Wheeler and G.H. Thorgaard, 2014. Antipredator behavior QTL: differences in rainbow trout clonal lines derived from wild and hatchery populations. Behav. Genet. doi 10.1007/s10519-014-9663-9
Brunelli, J.P., J.M. Mallatt, R.F. Leary, M. Alfaqih, R.B. Phillips and G.H. Thorgaard, 2013. Y chromosome phylogeny for cutthroat trout (Oncorhynchus clarkii) subspecies is generally concordant with those of other markers. Mol. Phylo. Evol. 66: 592-602.
Christensen K.A., J.P. Brunelli, M.J. Lambert, J. DeKoning, R. Phillips and G.H. Thorgaard, 2013. Identification of single nucleotide polymorphisms from the transcriptome of an organism with a whole genome duplication. BMC Bioinformatics 14:325.
Robison, B.D. and G. H. Thorgaard, 2012. Prospects and pitfalls of clonal fishes in the post-genomic era. In: G. L. Fletcher and M. Rise, editors. Aquaculture Biotechnology. Wiley-Blackwell , pp. 55-67.
Miller, M.R., J.P. Brunelli, P.A. Wheeler, S.Liu, C.E. Rexroad III,Y. Palti, C.Q. Doe and G.H. Thorgaard, 2012. A conserved haplotype controls parallel adaptation in geographically distant salmonid populations. Mol. Ecol. 21: 237-249.
Hale, M.C. P. Xu,, J. Scardina, P.A. Wheeler, G.H. Thorgaard and K.M. Nichols, 2011. Differential gene expression in male and female rainbow trout embryos prior to the onset of gross morphological differentiation of the gonads. BMC Genomics 12:404.
Martin, K.E., C.A. Steele, J.P. Brunelli and G.H. Thorgaard,
2010. Mitochondrial variation and biogeographic history of Chinook
salmon. Trans. Amer. Fish. Soc. 139: 792-802.
Brunelli, J.P,, C.A. Steele and G. H. Thorgaard, 2010. Deep divergence and apparent sex-biased dispersal revealed by a Y-linked marker in rainbow trout. Mol. Phylo. Evol. 56: 983-990.
Young, W.P., K. Frenyea, P.A. Wheeler and G. H. Thorgaard, 2009. No increase in developmental deformities or fluctuating asymmetry in rainbow trout (Oncorhynchus mykiss) produced with cryopreserved sperm. Aquaculture 289: 13-18.
Alfaqih, M.A., J.P. Brunelli, R.E. Drew and G.H. Thorgaard, 2009. Mapping of five candidate sex-determining loci in rainbow trout (Oncorhynchus mykiss). BMC Genetics 10: 2.
Brunelli, J.P., K.J. Wertzler, K. Sundin, and G.H. Thorgaard, 2008. Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon. Genome 51: 739-748.
Nichols, K.M., A. Felip Edo, P.A. Wheeler, and G.H. Thorgaard, 2008. The genetic basis of smoltification-related traits in Oncorhynchus mykiss. Genetics 179: 1559-1575.
Drew, R.E., H. Schwabl, P.A. Wheeler and G.H. Thorgaard, 2007. Detection of QTL influencing cortisol levels in rainbow trout (Oncorhynchus mykiss). Aquaculture 272S1: S183-S194.
Komen, H. and G.H. Thorgaard, 2007. Androgenesis, gynogenesis and the production of clones in fishes: a review. Aquaculture 269: 150-173.
Brown, K.H., R.W. Lee, and G.H. Thorgaard, 2006. Use of androgenesis for estimating maternal and mitochondrial haplotype effects on development and oxygen consumption in rainbow trout, Oncorhynchus mykiss. Comp. Biochem. Physiol. B: 143: 415-421.
Sundin, K., K.H. Brown, R.E. Drew, K.M. Nichols, P.A. Wheeler and G.H. Thorgaard, 2005 Genetic analysis of a development rate QTL in backcrosses of clonal rainbow trout. Aquaculture 247: 75-83.
Felip, A., A. Fujiwara, W.P. Young, P.A. Wheeler, M. Noakes, R.B. Phillips and G.H. Thorgaard, 2004. Polymorphism and differentiation of rainbow trout Y chromosomes. Genome 47: 1105-1113.
Lucas, M.D. R.E. Drew, P.A Wheeler, P.A. Verrell and G.H Thorgaard, 2004. Behavioral differences among rainbow trout clonal lines. Behavior Genetics 34: 355-365.
Zimmerman, A.M, J.P. Evenhuis, G.H. Thorgaard and S.S. Ristow, 2004. A single major chromosomal region controls natural killer cell-like activity in rainbow trout. Immunogenetics 55: 825-835.
Thorgaard, G.H., G.S. Bailey, D. Williams, D.R. Buhler, S.L. Kaattari, S.S. Ristow, J.D. Hansen, J.R. Winton, J.L. Bartholomew, J.J. Nagler, P.J. Walsh, M.M. Vijayan, R.H. Devlin, R.W. Hardy, K.E. Overturf, W.P. Young, B.D. Robison, C. Rexroad III, Y. Palti, 2002. Status and opportunities for genomics research with rainbow trout. Comp. Biochem. Physiol. B., 133: 609-646.